https://huntfish.mdc.mo.gov/fishing/regulations
Number of poles and hooks
If you use more than 3 poles (or 2 poles on the Mississippi River) at any one time, the additional poles must be labeled with your full name and address or Conservation Number. Regardless of the method or number of poles, you may not use more than a total of 33 hooks at any one time; except on the Mississippi River the maximum is 50 hooks at one time. If fishing on the Mississippi River and on other Missouri waters at the same time, no more than 50 hooks may be used and not more than 33 on waters other than the Mississippi.
Hooks on trotlines must be staged at least 2 feet apart. Hooks on any type of line, as well as the line itself, must be attended every 24 hours or removed.